scpB [2018-07-03 12:13:15]

part of the [SW|condensin] complex, chromosomal origin condensation and segregation

locus: BSU23210

pI: 4.25

mw: 21.89 kDa

protein length: 197 aa

gene length: 591 bp

function: segregation of replication origins

product: DNA segregation and condensation protein

essential: yes

synonyms: ypuH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]

Gene

Coordinates: 2,425,248 → 2,425,841

Phenotypes of a mutant

• essential [Pubmed|24440399], non-essential according to [Pubmed|28189581]
• ''[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]

The protein

Catalyzed reaction/ biological activity

• negatively regulates binding of the [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA] NTD to the [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc] neck region [pubmed|28286005]

Protein family

• scpB family (according to Swiss-Prot)

Structure

• [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] (aa 1-183) [Pubmed|23353789]

[SW|Localization]

• nucleoid (Multiple) [Pubmed|16479537]
• the [SW|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|Smc])₂-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|ScpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|ScpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|ParB] to ''parS'' centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

Expression and Regulation

Operons

• genes: [gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
  description: [Pubmed|7934830]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• MGNA-A105 (ypuH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/105 NBRP B. subtilis, Japan]
• BKE23210 (Δ[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA, downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
• BKK23210 (Δ[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA, downstream forward: _UP4_TAGAAGTTTAATGAATCGCG

References

Reviews

• 22933559,22934648,24118085,25460803,26706151,29182912

Original publications

• 12100548,12065423,12421306,22385855,16479537,19450516,12897137,7934830,11948165,23353789,24440399,24440393,25071173,25557547,25951515,26253537,23475963,26295962,26725510,26904953,28189581,28154080,28286005,28689660